The No Animal Sign is a sign found in Donkey Kong Country 2: Diddy's Kong Quest, Donkey Kong Country 3: Dixie Kong's Double Trouble!, and the Donkey Kong Land games. This sign prevents certain Animal Buddies from proceeding to the end of a stage. Players can finish the stage with their animal, but the animal does not carry on to later stages. They are likely added to the latter two games for the introduction of new ways to finish the stage.
Animal Signs are often considered frustrating because Kongs cannot take their buddies where they could be useful in later stage segments. However, the signs are usually found near the end of a stage (with the extreme exception of the stage Animal Antics), and when crossed, the Animal Buddy turns into a prize depending on how difficult it is to get to that specific area. Prizes range from coins to Extra Life Balloons, and, in Parry's case, a Bonus Barrel.
- A notable difference between the Super Nintendo Entertainment System and Game Boy Advance versions of Donkey Kong Country 2 and Donkey Kong Country 3 is that when a No-Animal Sign is passed, a slight ping sound is made instead of a loud boom.